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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).
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Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).

Journal: Journal of Dairy Science

Article Title: Evaluating the in vitro efficacy of bovine lactoferrin products against SARS-CoV-2 variants of concern

doi: 10.3168/jds.2021-21247

Figure Lengend Snippet: Anti-SARS-CoV-2 activity of dairy products. (A) Dose-response curves for dairy products. Data shown are the mean ± SEM of 10-point, 2-fold dilution series (n = 3 replicates per condition). Curve fitting was performed using GraphPad Prism 9.0 using a semi-log 4-parameter variable slope model. (B) Representative images for efficacious samples (e.g., 11-20-014-04) and the viral control. Cell nuclei are shown in cyan; viral nucleocapsid protein is shown in magenta. Images shown were taken on a Yokogawa CV8000 high-content microscope at 20× magnification and colored using ImageJ (National Institutes of Health).

Article Snippet: Dose-response curves for this data were fitted in GraphPad Prism 9.0 (GraphPad Software) using a semi-log 4-parameter variable slope model.

Techniques: Activity Assay, Microscopy